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基于液晶的适体传感器在飞摩尔水平检测盐酸莱克多巴胺
Microchemical Journal
(
IF
4.9
)
Pub Date : 2021-09-22
, DOI:
10.1016/j.microc.2021.106861
Chaerin Kim
1
,
Chang-Hyun Jang
1
Affiliation
- Department of Chemistry, Gachon University, Seongnam-daero 1342, Sujeong-gu, Seongnam-si, Gyeonggi-do 13120, Republic of Korea
我们开发了一种基于液晶 (LC) 的适体传感器,通过分析 LC 的光学结构,在飞摩尔级检测盐酸莱克多巴胺 (RAC)。基于 LC 的传感器包含适体,该适体在水相和 LC 之间的界面处与西曲溴铵 (CTAB) 和 RAC 发生反应。自组装 CTAB 使 LC 垂直锚定在水/LC 界面上。CTAB 填充的水相和 LC 之间界面处 LC 的排序取决于 RAC 的浓度。适配体和 RAC 之间的特异性结合事件优于适配体与 CTAB 的静电相互作用,当 RAC 的浓度高于 100 fM 时,导致 LC 保持垂直方向。相对主要的静电相互作用扰乱了液晶的取向,当 RAC 的浓度低于 100 fM 时,导致 LC 从垂直到倾斜或平面的锚定转变。我们监测了 LC 的光学响应,这取决于它们的排序转变。使用这种适体传感器,在磷酸盐缓冲盐水中检测到约 0.03 pg/mL (100 fM) 的 RAC,在猪尿中检测到约 3.0 pg/mL (10 pM)。通过比较 LC 光学图像的平均灰度强度数据,可以定量解释 LC 光学纹理的变化。我们的结果表明,使用适配体的基于 LC 的生物传感器具有高灵敏度、选择性和可重复使用性。使用这种适体传感器,在磷酸盐缓冲盐水中检测到约 0.03 pg/mL (100 fM) 的 RAC,在猪尿中检测到约 3.0 pg/mL (10 pM)。通过比较 LC 光学图像的平均灰度强度数据,可以定量解释 LC 光学纹理的变化。我们的结果表明,使用适配体的基于 LC 的生物传感器具有高灵敏度、选择性和可重复使用性。使用这种适体传感器,在磷酸盐缓冲盐水中检测到约 0.03 pg/mL (100 fM) 的 RAC,在猪尿中检测到约 3.0 pg/mL (10 pM)。通过比较 LC 光学图像的平均灰度强度数据,可以定量解释 LC 光学纹理的变化。我们的结果表明,使用适配体的基于 LC 的生物传感器具有高灵敏度、选择性和可重复使用性。
"点击查看英文标题和摘要"
Liquid crystal-based aptasensor to detect ractopamine hydrochloride at a femtomolar level
We developed a liquid crystal (LC)-based aptasensor for the detection of ractopamine hydrochloride (RAC) at a femtomolar level by analyzing the optical texture of LCs. The LC-based sensor comprises an aptamer that reacts to cetrimonium bromide (CTAB) and RAC at the interface between the aqueous phase and LCs. The self-assembly CTAB caused LCs to anchor perpendicularly at the aqueous/LC interface. The ordering of the LCs at the interface between the CTAB-packed aqueous phase and LCs depends on the concentration of RAC. Specific binding events between the aptamers and RAC were superior to the electrostatic interactions of the aptamers with CTAB, causing the LCs to remain in the homeotropic orientation when the concentration of RAC was higher than 100 fM. The relatively predominant electrostatic interactions perturb the orientation of LCs, resulting in the anchoring transition of LCs from homeotropic to tilted or planar when the concentration of RAC was lower than 100 fM. We monitored the optical response of the LCs, which depends on their ordering transitions. RAC was detected down to approximately ∼ 0.03 pg/mL (100 fM) in phosphate-buffered saline and ∼ 3.0 pg/mL (10 pM) in pig urine using this aptasensor. The change in the optical texture of the LCs was quantitatively interpreted by comparing the averaged grayscale intensity data of the LC optical image. Our results indicate that the LC-based biosensor using aptamers has high sensitivity, selectivity, and reusability.
更新日期:2021-09-22